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J Virol. 2015 Jan 15;89(2):1156-67. doi: 10.1128/JVI.02671-14. Epub 2014 Nov 5.

Activation of the chicken type I interferon response by infectious bronchitis coronavirus.

Author information

  • 1Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands MSD Animal Health, Bioprocess Technology & Support, Boxmeer, The Netherlands.
  • 2Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands.
  • 3Avian Viral Diseases, The Pirbright Institute, Compton Laboratory, United Kingdom.
  • 4Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
  • 5MSD Animal Health, Bioprocess Technology & Support, Boxmeer, The Netherlands.
  • 6Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands maria.forlenza@wur.nl.

Abstract

Coronaviruses from both the Alphacoronavirus and Betacoronavirus genera interfere with the type I interferon (IFN) response in various ways, ensuring the limited activation of the IFN response in most cell types. Of the gammacoronaviruses that mainly infect birds, little is known about the activation of the host immune response. We show that the prototypical Gammacoronavirus, infectious bronchitis virus (IBV), induces a delayed activation of the IFN response in primary renal cells, tracheal epithelial cells, and a chicken cell line. In fact, Ifnβ expression is delayed with respect to the peak of viral replication and the accompanying accumulation of double-stranded RNA (dsRNA). In addition, we demonstrate that MDA5 is the primary sensor for Gammacoronavirus infections in chicken cells. Furthermore, we provide evidence that accessory proteins 3a and 3b of IBV modulate the response at the transcriptional and translational levels. Finally, we show that, despite the lack of activation of the IFN response during the early phase of IBV infection, the signaling of nonself dsRNA through both MDA5 and TLR3 remains intact in IBV-infected cells. Taken together, this study provides the first comprehensive analysis of host-virus interactions of a Gammacoronavirus with avian innate immune responses.

IMPORTANCE:

Our results demonstrate that IBV has evolved multiple strategies to avoid the activation of the type I interferon response. Taken together, the present study closes a gap in the understanding of host-IBV interaction and paves the way for further characterization of the mechanisms underlying immune evasion strategies as well as the pathogenesis of gammacoronaviruses.

PMID:
25378498
PMCID:
PMC4300645
DOI:
10.1128/JVI.02671-14
[PubMed - indexed for MEDLINE]
Free PMC Article
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