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Chem Commun (Camb). 2018 Jan 2;54(3):299-302. doi: 10.1039/c7cc07344e.

A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging.

Author information

1
Department of Chemistry, Graduate School of Science and Integrated Research Consortium on Chemical Sciences (IRCCS), Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan.

Abstract

The far-red emissive fluorescent probe CaPF-1 based on a phospha-fluorescein scaffold enables the detection of cytosolic calcium ions in living cells. The probe can be excited in the red region (λabs = 636 nm) and exhibits a sufficiently high fluorescence turn-on response in the far-red region (λem = 663 nm) upon complexation with calcium ions. The hydrophilic and anionic characteristics of this phospha-fluorescein fluorophore allowed the cytosolic localization of CaPF-1. Moreover, it was possible to visualize histamine-induced calcium oscillation in HeLa cells using CaPF-1.

PMID:
29239411
DOI:
10.1039/c7cc07344e
[Indexed for MEDLINE]

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