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Molecules. 2017 Feb 11;22(2). pii: E271. doi: 10.3390/molecules22020271.

A Protein Isolate from Moringa oleifera Leaves Has Hypoglycemic and Antioxidant Effects in Alloxan-Induced Diabetic Mice.

Author information

1
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. paulolevemir@gmail.com.
2
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. danisilas@yahoo.com.br.
3
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. jtaolive@ufc.br.
4
Department of Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. aurano@ufc.br.
5
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. bellaagiselly@gmail.com.
6
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. mirellasally@gmail.com.
7
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. davi@dbm.ufpb.br.
8
Department of Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, Brazil. davi@dbm.ufpb.br.
9
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. vianamp@hotmail.com.
10
Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza 60430-160, Brazil. flavia@ufc.br.
11
Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza 60430-160, Brazil. talita.cm@hotmail.com.
12
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. imvasco@ufc.br.

Abstract

Moringa oleifera has been used in traditional medicine to treat diabetes. However, few studies have been conducted to relate its antidiabetic properties to proteins. In this study, a leaf protein isolate was obtained from M. oleifera leaves, named Mo-LPI, and the hypoglycemic and antioxidant effects on alloxan-induced diabetic mice were assessed. Mo-LPI was obtained by aqueous extraction, ammonium sulphate precipitation and dialysis. The electrophoresis profile and proteolytic hydrolysis confirmed its protein nature. Mo-LPI showed hemagglutinating activity, cross-reaction with anti-insulin antibodies and precipitation after zinc addition. Single-dose intraperitoneal (i.p.) administration of Mo-LPI (500 mg/kg·bw) reduced the blood glucose level (reductions of 34.3%, 60.9% and 66.4% after 1, 3 and 5 h, respectively). The effect of Mo-LPI was also evidenced in the repeated dose test with a 56.2% reduction in the blood glucose level on the 7th day after i.p. administration. Mo-LPI did not stimulate insulin secretion in diabetic mice. Mo-LPI was also effective in reducing the oxidative stress in diabetic mice by a decrease in malondialdehyde level and increase in catalase activity. Mo-LPI (2500 mg/kg·bw) did not cause acute toxicity to mice. Mo-LPI is a promising alternative or complementary agent to treat diabetes.

KEYWORDS:

Moringa; antioxidant activity; diabetes therapy; hypoglycemic activity; plant protein

PMID:
28208654
PMCID:
PMC6155657
DOI:
10.3390/molecules22020271
[Indexed for MEDLINE]
Free PMC Article

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