Format

Send to

Choose Destination
  • Showing results for a modified protocol for the isolation of primary human hepatocytes with improved viability and function from normal and diseased human liver. Your search for A Modified Protocol for the Isolation of Primary Human Hepatocytes with Improved Viability and Function from Normal and Diseased Human Live retrieved no results.
Methods Mol Biol. 2017;1506:61-73.

A Modified Protocol for the Isolation of Primary Human Hepatocytes with Improved Viability and Function from Normal and Diseased Human Liver.

Author information

1
National Institute for Health Research (NIHR) Biomedical Research Unit and Centre for Liver Research, University of Birmingham, Birmingham, UK. davidbartlett1@nhs.net.
2
The Liver Unit, Queen Elizabeth Hospital Birmingham, Birmingham, UK. davidbartlett1@nhs.net.
3
National Institute for Health Research (NIHR) Biomedical Research Unit and Centre for Liver Research, University of Birmingham, Birmingham, UK. p.n.newsome@bham.ac.uk.
4
The Liver Unit, Queen Elizabeth Hospital Birmingham, Birmingham, UK. p.n.newsome@bham.ac.uk.

Abstract

Successful hepatocyte isolation is critical for continued development of cellular transplantation. However, most tissue available for research is from diseased liver and the results of hepatocyte isolation from such tissue are inferior compared to normal tissue. Here we describe a modified method, combining the use of Liberase and N-acetylcysteine (NAC), for the isolation of primary human hepatocytes with high viability from normal and diseased liver.

KEYWORDS:

Hepatocyte isolation; Hepatocyte viability; Human hepatocyte; Liberase; N-Acetylcysteine

PMID:
27830545
DOI:
10.1007/978-1-4939-6506-9_4
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center