Cloning and characterization of DYRK1B, a novel member of the DYRK family of protein kinases

Biochem Biophys Res Commun. 1999 Jan 19;254(2):474-9. doi: 10.1006/bbrc.1998.9967.

Abstract

The DYRK1A gene on human chromosome 21 encodes a protein kinase presumed to be involved in the pathogenesis of mental retardation in Down's syndrome. Here we describe a highly similar homolog, DYRK1B, which is, in contrast to DYRK1A, predominately expressed in muscle and testis. The human DYRK1B gene was mapped to chromosome 19 (19q12-13.11) by radiation hybrid analysis. The amino acid sequences of DYRK1A and DYRK1B are 84% identical in the N-terminus and the catalytic domain but show no extended sequence similarity in the C-terminal region. DYRK1B contains all motifs characteristic for the DYRK family of protein kinases. In addition, the sequence comprises a bipartite nuclear localization motif. A green fluorescent protein (GFP) fusion protein of DYRK1B was found mainly in the nucleus of transfected COS-7 cells. These data suggest that DYRK1B is a muscle- and testis-specific isoform of DYRK1A and is involved in the regulation of nuclear functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • COS Cells
  • Cell Nucleus / metabolism
  • Chromosome Mapping
  • Chromosomes, Human, Pair 19*
  • Cloning, Molecular
  • Dyrk Kinases
  • Green Fluorescent Proteins
  • Humans
  • Luminescent Proteins / biosynthesis
  • Male
  • Molecular Sequence Data
  • Muscle, Skeletal / enzymology
  • Protein Kinases / biosynthesis
  • Protein Kinases / chemistry
  • Protein Kinases / genetics*
  • Protein Serine-Threonine Kinases*
  • Protein-Tyrosine Kinases*
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Testis / enzymology
  • Transfection

Substances

  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Green Fluorescent Proteins
  • Protein Kinases
  • Protein-Tyrosine Kinases
  • Protein Serine-Threonine Kinases

Associated data

  • GENBANK/Y17999
  • GENBANK/Y18280