Abstract
The mitochondrial single-stranded DNA-binding protein (SSB) encoded by anuclear gene, RIM1, is a homolog of Escherichia coli SSB. The addition of glucose decreased the amount of RIM1-mRNA in cells growing in a glycerol medium, but increased the amount of the immature RIM1-mRNA. The changes in the amounts of both mature and immature RIM1-mRNAs were dependent on SRN1/REG1/HEX2, a gene relating to pre-mRNA-splicing and glucose repression. These observations suggest that the expression of the mitochondrial SSB is regulated, at least in part, by pre-mRNA splicing under the control of glucose repression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Culture Media / pharmacology
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DNA, Single-Stranded / genetics
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DNA, Single-Stranded / metabolism
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DNA-Binding Proteins / genetics*
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Fungal Proteins / genetics*
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Gene Expression Regulation, Fungal / drug effects
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Genes, Fungal / genetics*
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Glucose / pharmacology*
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Mitochondria / genetics
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Mitochondria / metabolism
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Mutation
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Phosphoprotein Phosphatases*
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Protein Phosphatase 1
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RNA Precursors / drug effects
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RNA Precursors / genetics
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RNA Precursors / metabolism
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RNA Splicing
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RNA, Messenger / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Repressor Proteins
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Reverse Transcriptase Polymerase Chain Reaction
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Saccharomyces cerevisiae / drug effects*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
Substances
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Culture Media
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DNA, Single-Stranded
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DNA-Binding Proteins
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Fungal Proteins
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RIM101 protein, S cerevisiae
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RNA Precursors
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RNA, Messenger
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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Phosphoprotein Phosphatases
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Protein Phosphatase 1
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REG1 protein, S cerevisiae
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Glucose