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Nucleic Acids Res. 1998 Aug 15;26(16):3862-4.

In situ detection of protein-DNA interactions in filamentous fungi by in vivo footprinting.

Author information

1
IBTM, Technical University Vienna, Getreidemarkt 9/172-5, A-1060 Vienna, Austria, Institut für Biochemie,Vienna Biocenter, Dr. Bohr Gasse 9, A-1030 Vienna, Austria.

Abstract

The method described here allows the detection of protein-DNA interactions in vivo in filamentous fungi. We outline culture conditions and conditions of in vivo methylation that permit uniform modification of all cells in an apically growing, non-uniform organism, and subsequent visualization of protected areas by ligation-mediated PCR.

PMID:
9685506
PMCID:
PMC147760
DOI:
10.1093/nar/26.16.3862
[Indexed for MEDLINE]
Free PMC Article

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