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J Virol Methods. 1997 Jan;63(1-2):9-16.

A primer pair for amplifying part of the genome of all potyvirids by RT-PCR.

Author information

1
Research School of Biological Sciences, Australian National University, Canberra. gibbs@rsbs-central.anu.edu.au

Erratum in

  • J Virol Methods 1997 May;65(2):317.

Abstract

Sequence analysis was used to design a pair of degenerate oligonucleotide primers that amplified a 1.6-2.1 kbp fragment from the 3' end of the genome (virion protein gene and part of the NIb gene) of 17 species of the Potyviridae ('potyvirids'); 11 potyviruses, 2 bymoviruses, 2 macluraviruses, an ipomovirus and a rymovirus. The 'potyvirid primer 1' hybridizes to the 3' terminal poly-A region of the genome, and 'potyvirid primer 2' to the genomic region encoding the-GNNSGQ-motif of the NIb protein. Database searches showed that the potyvirid 2 primer is specific for potyvirids. Associated analyses indicated that the published amino acid sequence of part of the wheat streak mosaic rymovirus NIb protein is probably incorrect in part.

PMID:
9015271
DOI:
10.1016/s0166-0934(96)02103-9
[Indexed for MEDLINE]

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