Abstract
We have identified a cDNA clone for human cytidine deaminase (EC 3.5.4.5) during an investigation which aimed at cloning novel gene expression products related to monocyte/macrophage differentiation. The derived amino acid sequence of the clone comprises 145 residues yielding a molecular mass for the polypeptide of 16.1 kDa and exhibits a nearly 50% homology to cytidine deaminase from Bacillus subtilis. Cytidine deaminase activity of the cloned sequence could be demonstrated in a prokaryotic expression system. The mRNA is highly expressed in granulocytes while expression is very low in fibroblasts, chondrocytes, monocytes, and T- as well as B-cell lines. The mRNA can be induced in monocytes, the monocytoid cell line U937 and the myeloblastic line HL 60 by the differentiation inducer calcitriol.
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / enzymology
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Base Sequence
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Biomarkers
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Calcitriol / pharmacology
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Cell Differentiation*
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Cell Line
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Cells, Cultured
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Cloning, Molecular
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Cytidine Deaminase / analysis
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Cytidine Deaminase / genetics*
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Cytidine Deaminase / metabolism*
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DNA / genetics*
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Library
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Granulocytes / cytology*
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Granulocytes / enzymology
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Humans
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Macrophages / cytology*
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Macrophages / enzymology
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Molecular Sequence Data
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Monocytes / cytology*
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Monocytes / enzymology
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
Substances
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Biomarkers
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RNA, Messenger
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DNA
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Cytidine Deaminase
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Calcitriol
Associated data
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GENBANK/D13510
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GENBANK/S52873
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GENBANK/S64629
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GENBANK/S64630
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GENBANK/S64631
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GENBANK/S64632
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GENBANK/S64635
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GENBANK/S64636
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GENBANK/S64637
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GENBANK/U07167