Various cytokines modulate ICAM-1 shedding on melanoma- and CTCL-derived cell lines: inverse regulation of ICAM-1 shedding in a Sézary cell line by interferon-gamma

Dermatology. 1994;189(2):120-4. doi: 10.1159/000246813.

Abstract

Background: Since soluble intercellular adhesion molecules 1 (sICAM-1) retain their ability to bind to their ligand, they can interfere in cell-mediated immunosurveillance.

Objective: We studied the impact of various cytokines on ICAM-1 release of several tumor cell lines.

Methods: Two melanoma cell lines (M19, M26), 1 B lymphoblastoid cell line (Daudi), 1 erythroleukemia cell line (K562), 1 Sézary-cell-derived cell line (SeAx), 1 cell line derived from a mycosis fungoides lesion (MyLa) and normal peripheral blood mononuclear cells (PBMC) were grown in the presence of interferon gamma (IFN-gamma), IFN-alpha, interleukin-2 (IL-2), IL-6 and tumor necrosis factor alpha (TNF-alpha) in various concentrations. ICAM-1 release into the supernatant was measured after 24 and 48 h using an enzyme-linked immunosorbent assay (ELISA).

Results: PBMC and the B lymphoblastoid cell line did not shed detectable amounts of sICAM-1. In all other cell lines, ICAM-1 shedding was found with and without cytokine stimulation. In all cell cell lines except the CTCL-derived one, ICAM-1 shedding was marginally affected by IFN-alpha, IL-2 and IL-6. TNF-alpha and IFN-gamma enhanced the shedding in a time- and dose-dependent manner. In the SeAx line, IL-2 and IFN-gamma inhibited ICAM-1 release. By contrast, TNF-alpha and IL-6 enhanced it. The CTCL-derived MyLa responded to IFN-alpha with a dose-dependent increase in ICAM-1 shedding. All other cytokines had marginal influence.

Conclusion: Cytokine-modulated ICAM-1 shedding shows quantitative and qualitative differences in the investigated cell lines. This might have implications for the pathophysiology of cutaneous malignancies and their susceptibility to immunotherapies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / biosynthesis*
  • Cell Adhesion Molecules / biosynthesis*
  • Cell Migration Inhibition
  • Dose-Response Relationship, Drug
  • Humans
  • Intercellular Adhesion Molecule-1
  • Interferon-alpha / pharmacology*
  • Interferon-gamma / pharmacology*
  • Interleukin-2 / pharmacology*
  • Interleukin-6 / pharmacology*
  • Leukemia, Erythroblastic, Acute / metabolism*
  • Leukemia, Erythroblastic, Acute / pathology
  • Melanoma / metabolism*
  • Melanoma / pathology
  • Mycosis Fungoides / metabolism*
  • Mycosis Fungoides / pathology
  • Sezary Syndrome / metabolism*
  • Sezary Syndrome / pathology
  • Skin Neoplasms / metabolism*
  • Skin Neoplasms / pathology
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology*

Substances

  • Antigens, CD
  • Cell Adhesion Molecules
  • Interferon-alpha
  • Interleukin-2
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Intercellular Adhesion Molecule-1
  • Interferon-gamma