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Neurobiology (Bp). 1994;2(1):5-20.

Patch-clamp study of membrane properties and GABA-activated currents of rat magnocellular supraoptic neurons in thin slice preparation.

Author information

1
Department of Neurology, Technical University, M√ľnchen, Germany.

Abstract

The magnocellular neurons of the rat supraoptic nucleus were investigated by using (a) the patch-clamp technique on thin brain slice preparations to demonstrate voltage- and GABA-activated ionic currents, and (b) immunohistochemistry to demonstrate the expression of the beta 2 and beta 3 subunits of the GABAA-receptor on their membrane surface and the contents of the neuropeptides vasopressin and oxytocin. During electrophysiological recording in the whole-cell mode neurons were stained with Lucifer Yellow and camera lucida drawings were made. Two types of neurons could be distinguished by their different K(+)-currents, an inactivating and a noninactivating type. All neurons had a fast Na+ inward current. GABAA-activated currents were characterized by investigation of their ionic conductance and by blocking experiments with the GABAA-antagonist bicuculline.

PMID:
7812298
[Indexed for MEDLINE]

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