Abstract
Changes in cytokines, intercellular cell-matrix adhesion molecules and integrins may influenced tumor cell invasion and metastases. This study described the distribution, pattern and intensity of cytokine TGFa, adhesion molecules CD 34 and CD 44 and integrins a2, a3, CD 29 (beta 1 chain) and CD 61 (beta 3 chain) in hepatocellular carcinoma (HCC), metastatic liver tumors and hepatic cirrhosis. Fresh snap-frozen tissue from 20 cases of HCC, 5 metastatic adenocarcinomas and 10 cirrhotic livers was studied immunohistochemically using available antibodies. The most intense staining of TGFa was found in metastatic adenocarcinoma, following by regenerating hepatocytes in cirrhotic liver and well differentiated HCC. Insignificant differences in activity of CD 34 in various pathologies, up-regulation of CD 44 in poorly differentiated HCC and down-regulation in metastatic tumors were found. All integrins studied showed down-regulation in poorly differentiated HCC, relatively high activity of a2, a3 and beta 1 in metastatic tumors and the presence of all integrins in cirrhotic liver.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / chemistry
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Adenocarcinoma / genetics
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Adenocarcinoma / pathology
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Adenocarcinoma / secondary*
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Antigens, CD / biosynthesis
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Antigens, CD / genetics
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Antigens, CD34
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Carcinoma, Hepatocellular / chemistry*
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Carcinoma, Hepatocellular / genetics
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Carcinoma, Hepatocellular / pathology
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Carrier Proteins / biosynthesis
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Carrier Proteins / genetics
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Cell Adhesion Molecules / biosynthesis*
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Cell Adhesion Molecules / genetics
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Gene Expression Regulation, Neoplastic*
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Humans
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Hyaluronan Receptors
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Integrin alpha2
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Integrin alpha3
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Integrin beta1
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Integrin beta3
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Integrins / biosynthesis*
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Integrins / genetics
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Liver Cirrhosis / metabolism*
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Liver Cirrhosis / pathology
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Liver Neoplasms / chemistry*
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Liver Neoplasms / genetics
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Liver Neoplasms / pathology
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Liver Neoplasms / secondary
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Receptors, Cell Surface / biosynthesis
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Receptors, Cell Surface / genetics
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Receptors, Lymphocyte Homing / biosynthesis
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Receptors, Lymphocyte Homing / genetics
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Transforming Growth Factor alpha / biosynthesis*
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Transforming Growth Factor alpha / genetics
Substances
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Antigens, CD
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Antigens, CD34
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Carrier Proteins
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Cell Adhesion Molecules
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Hyaluronan Receptors
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Integrin alpha2
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Integrin alpha3
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Integrin beta1
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Integrin beta3
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Integrins
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Neoplasm Proteins
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Receptors, Cell Surface
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Receptors, Lymphocyte Homing
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Transforming Growth Factor alpha