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Arch Virol. 1981;68(3-4):221-8.

Variables that may influence the alkaline sedimentation pattern of herpes simplex virus DNA.

Abstract

Herpes simplex virus (HSV) DNA sediments homogeneously on a neutral sucrose gradient but heterogeneously on an alkaline sucrose gradient. Several factors that may influence the alkaline sedimentation pattern of HSV DNA were examined: e.g., the host cell, cell density at time of infection, multiplicity of infection, and the starting material for HSV DNA purification (whether HSV-infected cells or cell-free virus). Based on alkaline sedimentation analysis, these factors appear to play little or no role in the amount of intact single-stranded HSV DNA observed.

PMID:
6268021
DOI:
10.1007/bf01314575
[Indexed for MEDLINE]

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