Immune, inflammatory, autophagic and DNA damage responses to long-term H₂O₂ exposure in different tissues of common carp (Cyprinus carpio)

Hydrogen peroxide (H₂O₂) is a stable reactive oxygen species (ROS) in aquatic environment, and high concentration of ambient H₂O₂ may directly or indirectly affect aquatic animal health. However, the response mechanism of fish to ambient H₂O₂ has not been well studied yet. Therefore, the aim of the study was to investigate the immune, inflammatory, autophagic and DNA damage responses to long-term H₂O₂ exposure in different tissues of common carp. The results showed that H₂O₂ exposure induced a significant immune response, with alterations in the levels of immune parameters including AKP, ACP, LZM, C3, HSP90 and HSP70 in different tissues. The inflammatory response evoked by H₂O₂ exposure was associated with the activations of TLRs and NF-κB (P65) in the majority of tested tissues. The autophagy process was significantly affected by H₂O₂ exposure, evidenced by the upregulations of the autophagy-related genes in liver, gills, muscle, intestines, heart and spleen and the downregulations in kidney. Meanwhile, the mRNA level of atm, a primary transducer of DNA damage response, was upregulated in liver, gills, intestines and spleen, and the DNA damage was evidenced by increased 8-OHdG level in intestines after H₂O₂ exposure. Moreover, cell cycle regulation-related genes, including cyclin A1, B and/or E1, highly expressed in all tested tissues except heart after H₂O₂ exposure. Interestingly, IBR analysis exhibited that immune, inflammatory, autophagic and DNA damage responses to H₂O₂ exposure were in a dose-dependent and tissue-specific manner. These data may contribute to understanding H₂O₂ toxicity for fish and assessing potential risk of H₂O₂ in aquatic environment.