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Eur J Biochem. 1985 May 15;149(1):79-83.

31P nuclear magnetic resonance studies of crayfish (Orconectes virilis). The use of inversion spin transfer to monitor enzyme kinetics in vivo.


31P nuclear-magnetic resonance (NMR) has been used to observed in vivo the steady-state levels of phosphorus-containing metabolites in the crayfish Orconectes virilis and the intracellular pH of the abdominal muscle was determined. Measurement of spin-lattice relaxation times and spin transfer experiments have enabled calculation of unidirectional rate constants and activation energies for the arginine kinase reaction in vivo.

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