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Cell Rep. 2019 Dec 17;29(12):4186-4199.e3. doi: 10.1016/j.celrep.2019.11.051.

The Arabidopsis Transcriptome Responds Specifically and Dynamically to High Light Stress.

Author information

1
Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
2
Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Institute of Nuclear Agricultural Sciences, Key Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Zhejiang Province, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China. Electronic address: xbzhao@zju.edu.cn.
3
Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA. Electronic address: chory@salk.edu.

Abstract

The dynamic and specific transcriptome for high light (HL) stress in plants is poorly understood because heat has confounded previous studies. Here, we perform an in-depth temporal responsive transcriptome analysis and identify the core HL-responsive genes. By eliminating the effect of heat, we uncover a set of genes specifically regulated by high-intensity light-driven signaling. We find that 79% of HL-responsive genes restore their expression to baseline within a 14-h recovery period. Our study reveals that plants respond to HL through dynamic regulation of hormones, particularly abscisic acid (ABA), photosynthesis, and phenylpropanoid pathway genes. Blue/UV-A photoreceptors and phytochrome-interacting factor (PIF) genes are also responsive to HL. We further show that ABA biosynthesis-defective mutant nced3nced5, as well as pif4, pif5, pif4,5, and pif1,3,4,5 mutants, are hypersensitive to HL. Our study presents the dynamic and specific high-intensity light-driven transcriptional landscape in plants during HL stress.

KEYWORDS:

ABA; Arabidopsis; BBX; PIF; SAUR; high light; time course; transcriptome

PMID:
31851942
DOI:
10.1016/j.celrep.2019.11.051
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