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Front Biosci (Landmark Ed). 2020 Jan 1;25:710-721.

ADAR1 promotes HBV replication through its deaminase domain.

Author information

1
Department of Microbiology, College of Basic Medical Sciences/Molecular Medicine and Tumor Research Center, Chongqing Medical University, Chongqing 400016, China.
2
Department of Infectious Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
3
ffiliated hospital of chengde medical college.
4
Department of Clinical Laboratory, The First Hospital of Xi\'an, Xi\'an 710002, China.
5
Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
6
Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China, tuzeng@cqmu.edu.cn.

Abstract

Adenosine deaminase acting on RNA1 (ADAR1) is an RNA editing enzyme that modulates the replication of several viruses. Here, we provide evidence showing that ADAR1 stimulates hepatitis B virus (HBV) DNA replication in hepatocellular carcinoma cell lines that are transiently or stably-transfected with HBV. We show that overexpression of ADAR1 promotes the replication of all four HBV genotypes (A, B, C, and D). Furthermore, analysis by mutagenesis shows that RNA editing region, and to a lesser content, RNA binding region of ADAR is responsible for the promotion of replication. Together, these data show that ADAR1 stimulates HBV replication.

PMID:
31585913

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