Format

Send to

Choose Destination
Diagn Microbiol Infect Dis. 2019 Jun 24:114858. doi: 10.1016/j.diagmicrobio.2019.06.010. [Epub ahead of print]

INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex.

Author information

1
Department of Medical Microbiology, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands. Electronic address: t.vandenbroek-2@umcutrecht.nl.
2
Department of Oral and Maxillofacial Surgery, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands.
3
Department of Medical Microbiology, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands; Department of Medical Microbiology, Haaglanden Medical Center, 2501, CK, The Hague, The Netherlands.
4
Department of Medical Microbiology, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands.
5
National Institute for Public Health and the Environment (RIVM), 3720, BA, Bilthoven, The Netherlands.
6
Radboudumc Center for Infectious Diseases, Department of Medical Microbiology, Radboud University Medical Center, 6500, HB, Nijmegen, The Netherlands.

Abstract

Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.

KEYWORDS:

Case report; HSP65 sequencing; INNO-LiPA; Mycobacterium fortuitum complex; Mycobacterium smegmatis

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center