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Sci Rep. 2019 Jul 8;9(1):9885. doi: 10.1038/s41598-019-46086-y.

Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers.

Author information

1
Department of Pathology, Wrocław Medical University, Marcinkowskiego 1, 50-368, Wrocław, Poland. kamila.dus-szachniewicz@umed.wroc.pl.
2
Department of Optics and Photonics, Wrocław University of Science and Technology, Faculty of Fundamental Problems of Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.
3
Department of Pathology, Wrocław Medical University, Marcinkowskiego 1, 50-368, Wrocław, Poland.
4
Department of Statistics, Wrocław University of Economics, Komandorska 118/120, 53-345, Wrocław, Poland.
5
2nd Department of General and Oncological Surgery, Wrocław Medical University, Borowska 213, 50-556, Wrocław, Poland.
6
Division of Pathology, Sokołowski Hospital Wałbrzych, Sokołowskiego 4, 58-309, Wałbrzych, Poland.
7
Flow Cytometry Laboratory, Department of Pathology and Laboratory Diagnostics, Maria Sklodowska-Curie Institute-Oncology Centre, Wilhelma Konrada Roentgena 5, 02-781, Warsaw, Poland.

Abstract

We have adapted a non-invasive method based on optical tweezers technology to differentiate between the normal B-cells and the B-cell non-Hodgkin lymphoma (B-NHL) cells derived from clinical samples. Our approach bases on the nascent adhesion between an individual B-cell and a mesenchymal stromal cell. In this study, a single B-cell was trapped and optically seeded on a mesenchymal stromal cell and kept in a direct contact with it until a stable connection between the cells was formed in time scale. This approach allowed us to avoid the introduction of any exogenous beads or chemicals into the experimental setup which would have affected the cell-to-cell adhesion. Here, we have provided new evidence that aberrant adhesive properties found in transformed B-cells are related to malignant neoplasia. We have demonstrated that the mean time required for establishing adhesive interactions between an individual normal B-cell and a mesenchymal stromal cell was 26.7 ± 16.6 s, while for lymphoma cell it was 208.8 ± 102.3 s, p < 0.001. The contact time for adhesion to occur ranged from 5 to 90 s and from 60 to 480 s for normal B-cells and lymphoma cells, respectively. This method for optically controlled cell-to-cell adhesion in time scale is beneficial to the successful differentiation of pathological cells from normal B-cells within the fine needle aspiration biopsy of a clinical sample. Additionally, variations in time-dependent adhesion among subtypes of B-NHL, established here by the optical trapping, confirm earlier results pertaining to cell heterogeneity.

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