Objective: The urine concentrations of phthalate metabolites were used to estimate the cumulative risk assessment in preschool children in Ma'anshan of Anhui province. Methods: Based on the China-Anhui Birth Cohort, the demographic information and urine samples of 3 743 children were collected in Ma'anshan from April 2014 to April 2015. The concentrations of 7 metabolites' [monomethyl phthalate (MMP), monoethyl phthalate (MEP), monobutyl phthalate (MBP), monobenzyl phthalate (MBzP), mono (2-ethylhexyl) phthalate (MEHP), mono (2-ethyl- 5-oxohexyl) phthalate (MEOHP) and mono (2-ethyl-5-hydroxyhexyl) phthalate (MEHHP)] of 5 phthalates [dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), butyl benzyl phthalate (BBzP), and di (2-ethylhexyl) phthalate (DEHP)] in the urine samples of the children were measured by solid-phase extration-triple quadrupole high performance liquid chromatography-tandem mass spectrometry-isotope method. In addition, the estimated daily intakes (EDIs) of 5 phthalates were calculated according to the metabolites' concentrations. Cumulative risk assessment was performed using hazard quotient (HQ) and hazard index (HI) methods. Results: The M (Q(R)) of seven metabolite concentrations were 29.58 (18.69-48.26), 26.65 (13.44-56.09), 256.86 (150.99-438.51), 0.12 (0.04-0.32), 6.27 (3.71-11.13), 17.94 (11.94-28.42) and 24.80 (16.05-40.32) μg/g creatinine, respectively. For the EDIs of 5 phthalates, DBP ranked first, followed by DEHP, DMP, DEP and BBzP with the M (Q(R)) of 7.54 (4.41-12.85), 3.35 (2.20-5.42), 0.75 (0.47-1.24), 0.71 (0.36-1.52) and 0.003 (0.001-0.009) μg/(kg·d), respectively. The HQ and HI varied with age, gender and sampling season, the differences were significant (P<0.05). Conclusions: These results indicated that risk of cumulative exposure to phthalates was high in preschool children aged 3-6 years in Ma'anshan. Age, gender and sampling season were influencing factors.
目的： 通过检测尿中邻苯二甲酸酯（PAEs）代谢物浓度，评估安徽省马鞍山市学龄前儿童累积暴露的健康风险。 方法： 基于中国-安徽出生队列，2014年4月至2015年4月在安徽省马鞍山市采集3 743名儿童的基本信息和随机尿样。采用固相萃取-三重四级杆高效液相色谱串联质谱-同位素稀释法检测尿中5种PAEs[邻苯二甲酸二甲酯（DMP）；邻苯二甲酸二乙酯（DEP）和邻苯二甲酸二丁酯（DBP）；邻苯二甲酸丁苄酯（BBzP）；邻苯二甲酸单（2-乙基己）酯（DEHP）]的7种代谢物[邻苯二甲酸单甲酯（MMP）、邻苯二甲酸单乙酯（MEP）、邻苯二甲酸单丁酯（MBP）、邻苯二甲酸单苄酯（MBzP）、邻苯二甲酸单（2-乙基己）酯（MEHP）、邻苯二甲酸单（2-乙基-5-氧己基）酯（MEOHP）和邻苯二甲酸单（2-乙基-5-羟己基）酯（MEHHP）]浓度，根据代谢物浓度计算5种PAEs的每日估计暴露量。使用危害商（HQ）和危害指数（HI）法进行累积风险评估。 结果： 7种代谢物浓度M（Q(R)）分别为29.58（18.69~48.26）、26.65（13.44~56.09）、256.86（150.99~438.51）、0.12（0.04~0.32）、6.27（3.71~11.13）、17.94（11.94~28.42）和24.80（16.05~40.32）μg/g肌酐。5种PAEs的每日估计暴露量从高到低依次为DBP、DEHP、DMP、DEP和BBzP，M依次为7.54（4.41~12.85）、3.35（2.20~5.42）、0.75（0.47~1.24）、0.71（0.36~1.52）和0.003（0.001~0.009）μg/（kg·d）。不同月龄、性别和采样季节间的HQ和HI差异有统计学意义（P<0.05）。 结论： 马鞍山市3~6岁学龄前儿童PAEs累积暴露的健康风险较高，年龄、性别和季节是其影响因素。.
Keywords: Cumulative risk assessment; Hazard index; Phthalate; Preschool children.