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Plant Biotechnol J. 2019 May 26. doi: 10.1111/pbi.13174. [Epub ahead of print]

Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins.

Author information

1
RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Japan.
2
RIKEN Baton Zone Program, Tsurumi-ku, Yokohama, Japan.
3
euglena Co., Ltd., Tokyo, Japan.
4
Kihara Institute for Biological Research, Yokohama City University, Totsuka-ku, Yokohama, Japan.
5
Graduate School of Nanobioscience, Yokohama City University, Tsurumi-ku, Yokohama, Japan.
6
Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, Japan.

KEYWORDS:

Euglena gracilis ; CRISPR/Cas9; Microalgae; genome editing; knock-in; ribonucleoproteins; single-stranded oligodeoxynucleotides; transgene free

PMID:
31131534
DOI:
10.1111/pbi.13174
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