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Acta Cir Bras. 2019 May 6;34(4):e201900401. doi: 10.1590/s0102-865020190040000001.

Relations of neuropeptide Y and heme oxygenase-1 expressions with fetal brain injury in rats with intrahepatic cholestasis of pregnancy.

Author information

1
Doctor, Department of Obstetrics and Gynecology, The First Affiliated Hospital, Xi'an Jiao Tong University; and Department of Gynecology, Affiliated Hospital of Yan'an University, China. Design of the study, acquisition of data, technical procedures, final approval.
2
Master, Department of Anatomy, Yan'an University Medical College, China. Acquisition of data, technical procedures, manuscript preparation, final approval.
3
Bachelor, Department of Gynecology, Affiliated Hospital of Yan'an University, China. Acquisition of data, technical procedures, final approval.
4
Master, Affiliated Hospital of Yan'an University, China. Acquisition of data, technical procedures, final approval.
5
Master, Department of Obstetrics and Gynecology, The First Affiliated Hospital, Chongqing Medical University, China. Statistical analysis, manuscript preparation, final approval.
6
Doctor, Department of Obstetrics and Gynecology, The First Affiliated Hospital, Xi'an Jiao Tong University, China. Design of the study, manuscript preparation, final approval.

Abstract

PURPOSE:

To investigate the relations of neuropeptide Y (NPY) and heme oxygenase-1 (HO-1) expressions with fetal brain injury in rats with intrahepatic cholestasis of pregnancy (ICP).

METHODS:

Sixty rats pregnant for 15 days were randomly divided into experimental and control groups. The ICP model was established in experimental group. On the 21st day, the blood biochemical test, histopathological examination of pregnant rat liver and fetal brain tissues and immunohistochemical analysis of fetal rat brain tissues were performed.

RESULTS:

On the 21st day, the alanineaminotransferase, aspartate aminotransferase and total bile acid levels in experimental group were significantly higher than control group (P<0.01). Compared with control group, there was obvious vacuolar degeneration in pregnant rat liver tissue and fetal brain tissue in experimental group. NPY expression in fetal brain tissue was negative in control group and positive in experimental group. HO-1 expression in fetal brain tissue was strongly positive in control group and positive in experimental group. There was significant difference of immunohistochemical staining optical density between two groups (P<0.01).

CONCLUSION:

In fetal brain of ICP rats, the NPY expression is increased, and the HO-1 expression is decreased, which may be related to the fetal brain injury.

PMID:
31066785
PMCID:
PMC6583935
DOI:
10.1590/s0102-865020190040000001
[Indexed for MEDLINE]
Free PMC Article

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