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Nat Methods. 2019 Apr;16(4):323-325. doi: 10.1038/s41592-019-0361-7. Epub 2019 Mar 28.

Single-cell chromatin immunocleavage sequencing (scChIC-seq) to profile histone modification.

Author information

1
Laboratory of Epigenome Biology, Systems Biology Center, NHLBI, NIH, Bethesda, MD, USA.
2
National Institute of Health Sciences, Kawasaki, Japan.
3
Department of Pathophysiology and High Altitude Pathology, Third Military Medical University, Chongqing, China.
4
Department of Pathophysiology and High Altitude Pathology, Third Military Medical University, Chongqing, China. nibingxi@126.com.
5
Laboratory of Epigenome Biology, Systems Biology Center, NHLBI, NIH, Bethesda, MD, USA. zhaok@nhlbi.nih.gov.

Abstract

Our method for analyzing histone modifications, scChIC-seq (single-cell chromatin immunocleavage sequencing), involves targeting of the micrococcal nuclease (MNase) to a histone mark of choice by tethering to a specific antibody. Cleaved target sites are then selectively PCR amplified. We show that scChIC-seq reliably detects H3K4me3 and H3K27me3 target sites in single human white blood cells. The resulting data are used for clustering of blood cell types.

PMID:
30923384
DOI:
10.1038/s41592-019-0361-7
[Indexed for MEDLINE]

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