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RNA. 2019 Jun;25(6):713-726. doi: 10.1261/rna.069625.118. Epub 2019 Mar 20.

Activation of innate immunity by mitochondrial dsRNA in mouse cells lacking p53 protein.

Author information

1
CEITEC Masaryk University, 625 00 Brno, Czech Republic.
2
National Centre for Biomolecular Research, Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic.
#
Contributed equally

Abstract

Viral and cellular double-stranded RNA (dsRNA) is recognized by cytosolic innate immune sensors, including RIG-I-like receptors. Some cytoplasmic dsRNA is commonly present in cells, and one source is mitochondrial dsRNA, which results from bidirectional transcription of mitochondrial DNA (mtDNA). Here we demonstrate that Trp53 mutant mouse embryonic fibroblasts contain immune-stimulating endogenous dsRNA of mitochondrial origin. We show that the immune response induced by this dsRNA is mediated via RIG-I-like receptors and leads to the expression of type I interferon and proinflammatory cytokine genes. The mitochondrial dsRNA is cleaved by RNase L, which cleaves all cellular RNA including mitochondrial mRNAs, increasing activation of RIG-I-like receptors. When mitochondrial transcription is interrupted there is a subsequent decrease in this immune-stimulatory dsRNA. Our results reveal that the role of p53 in innate immunity is even more versatile and complex than previously anticipated. Our study, therefore, sheds new light on the role of endogenous RNA in diseases featuring aberrant immune responses.

KEYWORDS:

RNase L; innate immunity; mitochondrial dsRNA; p53

PMID:
30894411
PMCID:
PMC6521600
[Available on 2020-06-01]
DOI:
10.1261/rna.069625.118
[Indexed for MEDLINE]

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