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Proc Natl Acad Sci U S A. 2019 Apr 2;116(14):7053-7061. doi: 10.1073/pnas.1818485116. Epub 2019 Mar 14.

Transcriptome profiling of Plasmodium vivax in Saimiri monkeys identifies potential ligands for invasion.

Author information

1
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852; karthigayan.gunalan@nih.gov lmiller@niaid.nih.gov.
2
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852.
3
Rocky Mountain Laboratories, RTS Genomics Unit, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840.
4
Centers for Disease Control and Prevention, Atlanta, GA 30333.

Abstract

Unlike the case in Asia and Latin America, Plasmodium vivax infections are rare in sub-Saharan Africa due to the absence of the Duffy blood group antigen (Duffy antigen), the only known erythrocyte receptor for the P. vivax merozoite invasion ligand, Duffy binding protein 1 (DBP1). However, P. vivax infections have been documented in Duffy-negative individuals throughout Africa, suggesting that P. vivax may use ligands other than DBP1 to invade Duffy-negative erythrocytes through other receptors. To identify potential P. vivax ligands, we compared parasite gene expression in Saimiri and Aotus monkey erythrocytes infected with P. vivax Salvador I (Sal I). DBP1 binds Aotus but does not bind to Saimiri erythrocytes; thus, P. vivax Sal I must invade Saimiri erythrocytes independent of DBP1. Comparing RNA sequencing (RNAseq) data for late-stage infections in Saimiri and Aotus erythrocytes when invasion ligands are expressed, we identified genes that belong to tryptophan-rich antigen and merozoite surface protein 3 (MSP3) families that were more abundantly expressed in Saimiri infections compared with Aotus infections. These genes may encode potential ligands responsible for P. vivax infections of Duffy-negative Africans.

KEYWORDS:

Aotus; Duffy binding protein; Duffy blood group antigen; Plasmodium vivax; Saimiri

PMID:
30872477
PMCID:
PMC6452724
DOI:
10.1073/pnas.1818485116
[Indexed for MEDLINE]
Free PMC Article

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