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Front Biosci (Landmark Ed). 2019 Mar 1;24:1085-1096.

Interactions between human hemoglobin subunits and peroxiredoxin 2.

Author information

1
Laboratory of Hemoglobin, Baotou Medical College, 014060, Baotou, ChinaNo. 31, Jianshe Road, Donghe District, Baotou City, Inner Mongolia, China.
2
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Central Laboratory, Peking University Cancer Hospital & Institute, 100142, Beijing, China.
3
Laboratory of Hemoglobin, Baotou Medical College, 014060, Baotou, China No. 31, Jianshe Road, Donghe District, Baotou City, Inner Mongolia.
4
State Key Laboratory of Heavy Oil Processing and Department of Materials Science and Engineering, China University of Petroleum, 102249, Beijing, China.
5
Laboratory of Hemoglobin, Baotou Medical College, 014060, Baotou, ChinaNo. 31, Jianshe Road, Donghe District, Baotou City, Inner Mongolia.
6
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Central Laboratory, Peking University Cancer Hospital & Institute, 100142, Beijing, China,No. 52, Fucheng Road, Haidian District, Beijing City, Beijing.
7
Laboratory of Hemoglobin, Baotou Medical College, 014060, Baotou, ChinaNo. 31, Jianshe Road, Donghe District, Baotou City, Inner Mongolia, synmg@126.com.

Abstract

Red blood cells (RBCs) are exposed to exogenous reactive oxygen species in the circulatory system. To this end, the interactions between the different hemoglobin (Hb) subunits and peroxiredoxin 2, which is a ubiquitous member of the antioxidant enzymes that also controls the cytokine-induced peroxide levels, were assessed. We predicted by the increment of diversity with quadratic discriminant analysis (IDQD) that peroxiredoxin2 (Prx2) could interact with the hemoglobin alpha, beta and gamma subunits but not with the delta subunit. Coimmunoprecipitation (co-IP), electrospray ionization quadrupole time of flight (ESI-Q-TOF) mass spectrometry, Western blotting and X-ray absorption fine structure (XAFS) spectroscopy were performed to verify these predictions. The results showed that Prx2 was a member of the beta-globin immunoprecipitating complex that existed in hemoglobin A, hemolysate-hemoglobin A, hemoglobin A-hemoglobin A2, hemolysate-hemoglobin A-hemoglobin A2 and hemoglobin A2 but not in hemolysate-hemoglobin A2. Adding Prx2 to hemoglobin A altered the second shell of iron embedded in hemoglobin A. Therefore, Prx2 interacts with hemoglobin A (Alpha2Beta2) and hemoglobin F (Alpha2Gamma2) but not with hemoglobin A2 (Alpha2Delta2).

PMID:
30844732

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