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Front Biosci (Landmark Ed). 2019 Mar 1;24:616-627.

Sophocarpine inhibits the growth of gastric cancer cells via autophagy and apoptosis.

Author information

1
Department of General Surgery, Guangdong General Hospital, Guangdong Academy of Medical Science, Guangzhou, Guangdong 510000, China.
2
Department of general Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
3
Department of General Surgery, The Second Affiliated Hospital of Shantou University, 69 Dongxia North Road, Shantou, Guangdong 515100, China.
4
State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
5
Department of Surgery, Ruijin hospital Luwan Branch, Shanghai Jiao Tong University, School of Medicine, Shanghai 200020, China, 18917866321@163.com.

Abstract

Sophocarpine is one of the major ingredients of Sophorae flavescentis which could inhibits many kinds of cancers. However, the effect of sophocarpine on gastric cancer (GC) and the mechanism involved remain unknown. The present study aims to explore the effects of the sophocarpine on the proliferation and apoptosis of GC cells and elucidates the relevant molecular mechanisms. After treatment with sophocarpine, GC cells were evaluated on their proliferation, autophagy, cell cycle progress and apoptosis. The protein levels of LC3-I, LC3-II, Beclin, p62, PTEN, PI3K, p53, Bax, Bcl-2, AKT and p-AKT were detected by western blot. Sophocarpine inhibited the proliferation of GC cells both in vitro and in vivo dose-dependently. Sophocarpine not only caused cell apoptosis and cell cycle arrest in G0/G1 phase but also induced cell autophagy. Moreover, sophocarpine dose-dependently suppressed PI3K/AKT signaling pathway and activated apoptosis in gastric cancer cells. Thus, sophocarpine significantly inhibited the growth of GC cells through multiple mechanisms such as induction of autophagy, activation of cell apoptosis and down-regulation of cell survival signaling pathway.

PMID:
30844702

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