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Methods Mol Biol. 2019;1926:151-159. doi: 10.1007/978-1-4939-9021-4_13.

Gene Editing in 3D Cultured Nephron Progenitor Cell Lines.

Author information

1
Division of Nephrology and Hypertension, Department of Medicine and USC/UKRO Kidney Research Center, Keck School of Medicine of the University of Southern California, Los Angeles, CA, USA. zhongwei.li@med.usc.edu.
2
Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of the University of Southern California, Los Angeles, CA, USA. zhongwei.li@med.usc.edu.
3
Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
4
Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA. belmonte@salk.edu.

Abstract

During kidney development, Six2+ nephron progenitor cells (NPCs) generate nephrons, the functional units of the kidney. Isolating and expanding NPCs in vitro have enabled wide applications in both basic and translational research. Here we describe the methods to derive NPC lines from mouse embryonic kidneys in a 3D culture format as well as gene editing in the NPC lines.

KEYWORDS:

3D culture; CRISPR-Cas9; Gene editing; NPSR culture medium; Nephron progenitor cell (NPC)

PMID:
30742270
DOI:
10.1007/978-1-4939-9021-4_13
[Indexed for MEDLINE]

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