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Elife. 2019 Feb 8;8. pii: e42687. doi: 10.7554/eLife.42687.

Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping.

Author information

1
Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, United States.
2
Neuroscience and Cognitive Science Program, University of Maryland, College Park, United States.
3
Advanced Research Computing, Department of Computer Science, Virginia Polytechnic Institute and State University, Blacksburg, United States.
4
Postdoctoral Research Associate Training Program, National Institute of General Medical Sciences, Bethesda, United States.

Abstract

Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.

KEYWORDS:

Cre; Gal4; brain atlas; imaging; intersectional genetics; neuroscience; registration; zebrafish

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