Format

Send to

Choose Destination
Nat Commun. 2019 Jan 30;10(1):496. doi: 10.1038/s41467-019-08378-9.

Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells.

Author information

1
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 10094, China.
2
Fujian Provincial Key Laboratory of Reproductive Health Research, Medical College of Xiamen University, Xiamen, Fujian, 361102, China.
3
Colorado Center for Reproductive Medicine, Lone Tree, CO, 80124, USA.
4
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
5
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
6
Animal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
7
Salk Institute for Biological Studies, La Jolla, CA, 92037, USA.
8
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 10094, China. hanjy@cau.edu.cn.
9
Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100083, China. hanjy@cau.edu.cn.

Abstract

Spatially ordered embryo-like structures self-assembled from blastocyst-derived stem cells can be generated to mimic embryogenesis in vitro. However, the assembly system and developmental potential of such structures needs to be further studied. Here, we devise a nonadherent-suspension-shaking system to generate self-assembled embryo-like structures (ETX-embryoids) using mouse embryonic, trophoblast and extra-embryonic endoderm stem cells. When cultured together, the three cell types aggregate and sort into lineage-specific compartments. Signaling among these compartments results in molecular and morphogenic events that closely mimic those observed in wild-type embryos. These ETX-embryoids exhibit lumenogenesis, asymmetric patterns of gene expression for markers of mesoderm and primordial germ cell precursors, and formation of anterior visceral endoderm-like tissues. After transplantation into the pseudopregnant mouse uterus, ETX-embryoids efficiently initiate implantation and trigger the formation of decidual tissues. The ability of the three cell types to self-assemble into an embryo-like structure in vitro provides a powerful model system for studying embryogenesis.

PMID:
30700702
PMCID:
PMC6353907
DOI:
10.1038/s41467-019-08378-9
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center