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Cancer Immunol Immunother. 2019 Jan 25. doi: 10.1007/s00262-019-02302-2. [Epub ahead of print]

Deciphering myeloid-derived suppressor cells: isolation and markers in humans, mice and non-human primates.

Author information

1
MRC Centre for Reproductive Health, Queen's Medical Research Institute, The University of Edinburgh, 47 Little France Crescent, EH16 4TJ, Edinburgh, UK. Luca.cassetta@ed.ac.uk.
2
Centre for Immune Regulation, Department of Pathology, University of Oslo, Oslo University Hospital, Rikshospitalet, Oslo, Norway.
3
West German Cancer Center, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
4
Division of General Pathology, Department of Medicine, University of Verona, Verona, Italy.
5
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald, Insel Riems, Germany.
6
Faculty of Mathematics and Natural Sciences, University of Greifswald, Greifswald, Germany.
7
Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany.
8
Department of Microbiology and Immunology, Hollings Cancer Center, Medical University of South Carolina, Charleston, USA.
9
Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet, Stockholm, Sweden.
10
Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.
11
MRC Centre for Reproductive Health, Queen's Medical Research Institute, The University of Edinburgh, 47 Little France Crescent, EH16 4TJ, Edinburgh, UK.
12
Centre Hospitalier Universitaire, Pôle Biologie, INSERM, UMR U1236, Université Rennes 1, EFS Bretagne, Rennes, France.
13
Skin Cancer Unit, German Cancer Research Center (DKFZ), Heidelberg, Germany.
14
Department of Dermatology, Venereology and Allergology, University Medical Center Mannheim, Ruprecht-Karl University of Heidelberg, Mannheim, Germany.
15
Radiotherapy and OncoImmunology Laboratory, Department of Radiation Oncology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Geert Grooteplein 28, 6500 HB, Nijmegen, The Netherlands. Gosse.Adema@RadboudUmc.nl.

Abstract

In cancer, infection and inflammation, the immune system's function can be dysregulated. Instead of fighting disease, immune cells may increase pathology and suppress host-protective immune responses. Myeloid cells show high plasticity and adapt to changing conditions and pathological challenges. Despite their relevance in disease pathophysiology, the identity, heterogeneity and biology of myeloid cells is still poorly understood. We will focus on phenotypical and functional markers of one of the key myeloid regulatory subtypes, the myeloid derived suppressor cells (MDSC), in humans, mice and non-human primates. Technical issues regarding the isolation of the cells from tissues and blood, timing and sample handling of MDSC will be detailed. Localization of MDSC in a tissue context is of crucial importance and immunohistochemistry approaches for this purpose are discussed. A minimal antibody panel for MDSC research is provided as part of the Mye-EUNITER COST action. Strategies for the identification of additional markers applying state of the art technologies such as mass cytometry will be highlighted. Such marker sets can be used to study MDSC phenotypes across tissues, diseases as well as species and will be crucial to accelerate MDSC research in health and disease.

KEYWORDS:

Human; Mouse; Mye-EUNITER; Myeloid-derived suppressor cells; Non-human primates

PMID:
30684003
DOI:
10.1007/s00262-019-02302-2

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