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Future Microbiol. 2019 Jan 21. doi: 10.2217/fmb-2018-0203. [Epub ahead of print]

The fecal and mucosal microbiome in acute appendicitis patients: an observational study.

Author information

1
Department of Infectious Diseases & Immunity, Jessa Hospital, Hasselt, Belgium.
2
Faculty of Medicine & Life Sciences, Hasselt University, Hasselt, Belgium.
3
Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
4
Radboudumc Center for Infectious Diseases (RCI), Radboud University Medical Center, Nijmegen, The Netherlands.
5
School of Nutrition & Translational Research in Metabolism (NUTRIM) & School for Public Health & Primary Care (CAPHRI), Department of Medical Microbiology, Maastricht University Medical Center+, Maastricht, The Netherlands.
6
Department of Abdominal & Oncological Surgery, Jessa Hospital, Hasselt, Belgium.
7
Department of Medical Microbiology & Infection Control, VU University Medical Center, Amsterdam, The Netherlands.

Abstract

AIM:

We aimed to study the mucosal microbiota of the appendix in a prospective appendicitis cohort and to compare the fecal microbiota of patients and controls. We hypothesized that the microbiota may be associated with susceptibility to appendicitis.

PATIENTS & METHODS:

The fecal microbiota of 99 patients and 106 controls were characterized using 16S-23S intergenic spacer profiling. Richness, diversity and community structure were compared. The appendiceal microbiota from 90 patients was analyzed according to the severity of appendicitis.

RESULTS:

Overall fecal microbial richness and diversity were similar in patients and controls, yet richness and diversity within the group of Firmicutes, Actinobacteria, Fusobacteria and Verrucomicrobia phyla were lower in patients. Discriminant analyses could correctly classify patients and controls with fair accuracy. No differences were found according to severity in appendiceal or fecal microbiota.

CONCLUSION:

This study demonstrates differences in the composition of intestinal microbiota of appendicitis patients and healthy individuals.

KEYWORDS:

acute appendicitis; adult population; dysbiosis; intestinal microbiota; microbiome; prospective cohort study

PMID:
30663346
DOI:
10.2217/fmb-2018-0203

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