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Biol Trace Elem Res. 2019 Jan 19. doi: 10.1007/s12011-019-1642-9. [Epub ahead of print]

Decreased Expression of CHST-12, CHST-13, and UST in the Proximal Interphalangeal Joint Cartilage of School-Age Children with Kashin-Beck Disease: an Endemic Osteoarthritis in China Caused by Selenium Deficiency.

Guo Y1, Zhou Y1, Yan S2, Qu C3, Wang L1,4, Guo X1, Han J5,6,7.

Author information

1
Key laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.
2
Department of Ophthalmology, the First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.
3
Department of Integrative Medical Biology, Umeå University, 90187, Umeå, Sweden.
4
College of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.
5
Key laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China. bbbishop@126.com.
6
College of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China. bbbishop@126.com.
7
Shenzhen Institute, Xi'an Jiaotong University, Shenzhen, 518057, Guangzhou, People's Republic of China. bbbishop@126.com.

Abstract

The objective of this study is to investigate changes in the expression of enzymes involved in chondroitin sulfate (CS) sulfation in distal articular surface of proximal interphalangeal joint isolated from school-age children patients with Kashin-Beck disease (KBD), using normal children as controls. Articular cartilage samples were collected from four normal and four KBD children (7-12 years old), and these children were assigned to control and KBD groups. Hematoxylin and eosin (H&E), toluidine blue (TB), and immunohistochemical (IHC) stainings were utilized to evaluate changes in joint pathology and expression of enzymes involved in CS sulfation, including carbohydrate sulfotransferase 12 (CHST-12), carbohydrate sulfotransferase 13 (CHST-13), and uronyl 2-O-sulfotransferase (UST). The correspondence results were examined by semi-quantitative analysis. Compared with the control group, the KBD group showed the following: a significant decrease of total chondrocytes in superficial, middle, and deep layers and deposition of sulfated glycosaminoglycans in extracellular matrix of KBD cartilage were observed; positive staining chondrocytes of CHST-12, CHST-13, and UST were significantly less in superficial zone of KBD cartilage; and CHST-13 positive staining chondrocytes was reduced in deep zone of KBD cartilage. In contrast, the positive staining rates of CHST-12, CHST-13, and UST in KBD were significantly higher than those in the control group. The decreased expression of these enzymes and the physiologic compensatory reaction may be the signs of early-stage KBD. The alterations of CS structure modifying sulfotransferases in finger articular cartilage might play an important role in the onset and pathogenesis of school-age KBD children.

KEYWORDS:

Child cartilage; Chondroitin sulfate; Kashin–Beck disease; Selenium deficiency; Sulfation; Sulfotransferases

PMID:
30661165
DOI:
10.1007/s12011-019-1642-9

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