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Biosensors (Basel). 2019 Jan 9;9(1). pii: E10. doi: 10.3390/bios9010010.

A Bioelectronic System to Measure the Glycolytic Metabolism of Activated CD4+ T Cells.

Author information

1
Life Sciences Discipline, Burnet Institute, Melbourne, VIC 3001, Australia. suzanne.crowe@burnet.edu.au.
2
Department of Infectious Diseases, Monash University, Melbourne, VIC 3004, Australia. suzanne.crowe@burnet.edu.au.
3
Infectious Diseases Department, The Alfred hospital, Melbourne, VIC 3004, Australia. suzanne.crowe@burnet.edu.au.
4
Laboratory of Cell Technology, School of Food Science, Biotechnology and Development, Agricultural University of Athens, 11855 Athens, Greece. skin@aua.gr.
5
Department of Electrical and Electronics Engineering, microSENSES lab, University of West Attika, 12244 Athens, Greece. G.Kaltsas@uniwa.gr.
6
Life Sciences Discipline, Burnet Institute, Melbourne, VIC 3001, Australia. clovis.palmer@burnet.edu.au.
7
Department of Infectious Diseases, Monash University, Melbourne, VIC 3004, Australia. clovis.palmer@burnet.edu.au.

Abstract

The evaluation of glucose metabolic activity in immune cells is becoming an increasingly standard task in immunological research. In this study, we described a sensitive, inexpensive, and non-radioactive assay for the direct and rapid measurement of the metabolic activity of CD4+ T cells in culture. A portable, custom-built Cell Culture Metabolite Biosensor device was designed to measure the levels of acidification (a proxy for glycolysis) in cell-free CD4+ T cell culture media. In this assay, ex vivo activated CD4+ T cells were incubated in culture medium and mini electrodes were placed inside the cell free culture filtrates in 96-well plates. Using this technique, the inhibitors of glycolysis were shown to suppress acidification of the cell culture media, a response similar to that observed using a gold standard lactate assay kit. Our findings show that this innovative biosensor technology has potential for applications in metabolic research, where acquisition of sufficient cellular material for ex vivo analyses presents a substantial challenge.

KEYWORDS:

Glut1; HIV; T cells; bioelectronics; glycolysis; immunometabolism; metabolism; mitochondria

PMID:
30634392
DOI:
10.3390/bios9010010
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