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Turk J Ophthalmol. 2018 Dec 27;48(6):281-287. doi: 10.4274/tjo.50146.

Effects of Topical Thymoquinone in an Experimental Dry Eye Model

Author information

1
Adnan Menderes University Faculty of Medicine, Department of Ophthalmology, Aydın, Turkey
2
Yozgat City Hospital, Ophthalmology Clinic, Yozgat, Turkey
3
Adnan Menderes University Faculty of Medicine, Department of Pathology, Aydın, Turkey
4
Adnan Menderes University Faculty of Veterinary Medicine, Department of Basic Sciences of Veterinary Medicine, Division of Veterinary Physiology, Aydın, Turkey
5
Adnan Menderes University Faculty of Medicine, Department of Biochemistry, Aydın, Turkey
6
Adnan Menderes University Faculty of Medicine, Department of Hematology, Aydın, Turkey
7
Batıgöz Hospital, Ophthalmology Clinic, İzmir, Turkey

Abstract

Objectives:

To comparatively evaluate the effects of thymoquinone (TQ), the biologically active main component of volatile oil derived from Nigella sativa seeds, in an experimental dry eye model.

Materials and Methods:

A total of 36 BALB/c mice 10 weeks of age were used in the study. The mice were divided into 6 groups of 6 mice. Two groups were negative and positive controls, and the other 4 groups were treated with balanced salt solution, fluorometholone (FML), TQ, or vehicle (Tween80). After 1 week of treatment, the mice were killed and the eyes removed for histopathologic examination and cytokine analysis. Interleukin (IL)-1α tumor necrosis factor-α, interferon-γ, IL-2, IL-6, IL-10, and lactoferrin levels in the conjunctival tissue were measured by multiplex immunobead assay. The presence of inflammatory cells in ocular tissue samples were investigated by hematoxylin-eosin and periodic acid-Schiff staining. Inflammatory T cells containing CXT receptor in the conjunctiva were determined by flow cytometry.

Results:

FLML and TQ groups had less inflammatory cell density and more goblet cells compared to the other groups. High levels of IL-1α and IL-2 were found in the TQ group.

Conclusion:

TQ treatment was associated with reduced inflammation in pathological examination, but did not significant lower cytokine levels.

KEYWORDS:

Black seed oil; dry eye; thymoquinone

PMID:
30605933
PMCID:
PMC6330663
DOI:
10.4274/tjo.50146
[Indexed for MEDLINE]
Free PMC Article

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