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BMC Mol Biol. 2019 Jan 3;20(1):2. doi: 10.1186/s12867-018-0119-2.

Graphene oxide down-regulates genes of the oxidative phosphorylation complexes in a glioblastoma.

Author information

1
Department of Morphological Sciences, Warsaw University of Life Sciences, 02-787, Warsaw, Poland.
2
Department of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552, Jastrzebiec, Poland.
3
Department of Animal Nutrition and Biotechnology, Warsaw University of Life Sciences, 02-787, Warsaw, Poland.
4
Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106, Warsaw, Poland.
5
Department of Veterinary and Animal Sciences, University of Copenhagen, Groennegaardsvje 3, 1870, Frederiksberg, Denmark. ach@sund.ku.dk.

Abstract

BACKGROUND:

Recently different forms of nanographene were proposed as the material with high anticancer potential. However, the mechanism of the suppressive activity of the graphene on cancer development remains unclear. We examined the effect of oxygenated, reduced and pristine graphene on the gene expression in glioblastoma U87 cell line.

RESULTS:

Conducting microarrays and RT-qPCR analysis we explored that graphene oxide (rather than reduced graphene oxide and pristine graphene) down-regulates the mRNA expression of mitochondrial oxidative phosphorylation (OXPHOS) nuclear genes of complexes I, III, IV and V. The presented results provide first evidence for the hypothesis that the suppressed growth of GBM can be the consequence of down-regulation of OXPHOS protein expression and decreased ATP level.

CONCLUSIONS:

We suggest that changes in the expression of OXPHOS genes identified in our study may mediate the anti-proliferative and anti-migratory effects of graphene oxide in glioblastoma cells. However, further investigations with different cell lines, regarding expression, regulation and activity of OXPHOS genes identified in our study is necessary to elucidate the mechanism mediating the anti-proliferative and anti-migratory effects of graphene oxide in glioblastoma cells.

KEYWORDS:

Gene expression; Glioblastoma; Graphene; Graphene oxide; OXPHOS

PMID:
30602369
PMCID:
PMC6317254
DOI:
10.1186/s12867-018-0119-2
[Indexed for MEDLINE]
Free PMC Article

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