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Nat Methods. 2018 Dec;15(12):1045-1047. doi: 10.1038/s41592-018-0212-y. Epub 2018 Nov 30.

Efficient scarless genome editing in human pluripotent stem cells.

Author information

1
Department of Pediatrics, Stanford University, Stanford, CA, USA.
2
ReGen Med Division, BOCO Silicon Valley, Palo Alto, CA, USA.
3
Department of Genetics, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA, USA.
4
Department of Obstetrics and Gynecology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA, USA.
5
Department of Pediatrics, Stanford University, Stanford, CA, USA. mporteus@stanford.edu.

Abstract

Scarless genome editing in human pluripotent stem cells (hPSCs) represents a goal for both precise research applications and clinical translation of hPSC-derived therapies. Here we established a versatile and efficient method that combines CRISPR-Cas9-mediated homologous recombination with positive-negative selection of edited clones to generate scarless genetic changes in hPSCs.

PMID:
30504872
DOI:
10.1038/s41592-018-0212-y
[Indexed for MEDLINE]

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