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Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):E11837-E11846. doi: 10.1073/pnas.1809662115. Epub 2018 Nov 21.

TRPV1 pore turret dictates distinct DkTx and capsaicin gating.

Author information

1
The Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Ein Karem, 9112102 Jerusalem, Israel.
2
Theoretical Molecular Biophysics Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
3
Department of Physiology, The University of Tennessee Health Science Center, Memphis, TN 38163 vvasquez@uthsc.edu avip@ekmd.huji.ac.il.
4
The Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Ein Karem, 9112102 Jerusalem, Israel; vvasquez@uthsc.edu avip@ekmd.huji.ac.il.

Abstract

Many neurotoxins inflict pain by targeting receptors expressed on nociceptors, such as the polymodal cationic channel TRPV1. The tarantula double-knot toxin (DkTx) is a peptide with an atypical bivalent structure, providing it with the unique capability to lock TRPV1 in its open state and evoke an irreversible channel activation. Here, we describe a distinct gating mechanism of DkTx-evoked TRPV1 activation. Interestingly, DkTx evokes significantly smaller TRPV1 macroscopic currents than capsaicin, with a significantly lower unitary conductance. Accordingly, while capsaicin evokes aversive behaviors in TRPV1-transgenic Caenorhabditis elegans, DkTx fails to evoke such response at physiological concentrations. To determine the structural feature(s) responsible for this phenomenon, we engineered and evaluated a series of mutated toxins and TRPV1 channels. We found that elongating the DkTx linker, which connects its two knots, increases channel conductance compared with currents elicited by the native toxin. Importantly, deletion of the TRPV1 pore turret, a stretch of amino acids protruding out of the channel's outer pore region, is sufficient to produce both full conductance and aversive behaviors in response to DkTx. Interestingly, this deletion decreases the capsaicin-evoked channel activation. Taken together with structure modeling analysis, our results demonstrate that the TRPV1 pore turret restricts DkTx-mediated pore opening, probably through steric hindrance, limiting the current size and mitigating the evoked downstream physiological response. Overall, our findings reveal that DkTx and capsaicin elicit distinct TRPV1 gating mechanisms and subsequent pain responses. Our results also indicate that the TRPV1 pore turret regulates the mechanisms of channel gating and permeation.

KEYWORDS:

DkTx; TRPV1; capsaicin; pore turret; transgenic C. elegans

PMID:
30463948
PMCID:
PMC6294906
DOI:
10.1073/pnas.1809662115
[Indexed for MEDLINE]
Free PMC Article

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