Format

Send to

Choose Destination
Sci Rep. 2018 Nov 13;8(1):16731. doi: 10.1038/s41598-018-34589-z.

Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.

Author information

1
Institute for Medical Microbiology, Immunology, and Hygiene, Technische Universität München (TUM), Munich, Germany.
2
Juno Cell Therapeutics GmbH, A Celgene Company, Munich, Germany.
3
IBA GmbH, Göttingen, Germany.
4
National Center for Infection Research (DZIF), Munich, Germany.
5
Focus Group "Clinical Cell Processing and Purification," Institute for Advanced Study, TUM, Munich, Germany.
6
Klinik und Poliklinik für Frauenheilkunde, Technische Universität München (TUM), Munich, Germany.
7
Institute for Medical Microbiology, Immunology, and Hygiene, Technische Universität München (TUM), Munich, Germany. dirk.busch@tum.de.
8
National Center for Infection Research (DZIF), Munich, Germany. dirk.busch@tum.de.
9
Focus Group "Clinical Cell Processing and Purification," Institute for Advanced Study, TUM, Munich, Germany. dirk.busch@tum.de.

Abstract

We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent functionality of purified cells.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center