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Dev Cell. 2018 Nov 5;47(3):377-387.e4. doi: 10.1016/j.devcel.2018.10.013.

Oxidative Stress Orchestrates Cell Polarity to Promote Embryonic Wound Healing.

Author information

1
Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3G5, Canada; Ted Rogers Centre for Heart Research, Translational Biology and Engineering Program, University of Toronto, Toronto, ON M5G 1M1, Canada.
2
Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3G5, Canada.
3
Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3G5, Canada; Ted Rogers Centre for Heart Research, Translational Biology and Engineering Program, University of Toronto, Toronto, ON M5G 1M1, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada; Developmental and Stem Cell Biology Program, Hospital for Sick Children, Toronto, ON M5G 1X8, Canada. Electronic address: rodrigo.fernandez.gonzalez@utoronto.ca.

Abstract

Embryos have a striking ability to heal wounds rapidly and without scarring. Embryonic wound repair is a conserved process, driven by polarization of cell-cell junctions and the actomyosin cytoskeleton in the cells around the wound. However, the upstream signals that trigger cell polarization around wounds are unknown. We used quantitative in vivo microscopy in Drosophila and zebrafish embryos to identify reactive oxygen species (ROS) as a critical signal that orchestrates cell polarity around wounds. ROS promote trafficking of adherens junctions and accumulation of actin and myosin at the wound edge and are necessary for wound closure. We show that, in Drosophila, ROS drive wound healing in part through an ortholog of Src kinase, Src42A, which we identify as a redox sensor that promotes polarization of junctions and the cytoskeleton around wounds. We propose that ROS are a reparative signal that drives rapid embryonic wound healing in vertebrate and invertebrate species.

KEYWORDS:

cell polarity; cell-cell junctions; cytoskeletal dynamics; epithelial morphogenesis

PMID:
30399336
DOI:
10.1016/j.devcel.2018.10.013
[Indexed for MEDLINE]

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