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G3 (Bethesda). 2019 Jan 9;9(1):81-94. doi: 10.1534/g3.118.200726.

Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the Drosophila Mushroom Bodies.

Author information

1
Department of Anesthesiology, Stony Brook School of Medicine, Stony Brook, NY.
2
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA; National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD.
3
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY joshua.dubnau@stonybrook.edu Henry@cshl.edu.
4
Department of Anesthesiology, Stony Brook School of Medicine; Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, NY joshua.dubnau@stonybrook.edu Henry@cshl.edu.

Abstract

The insect mushroom body (MB) is a conserved brain structure that plays key roles in a diverse array of behaviors. The Drosophila melanogaster MB is the primary invertebrate model of neural circuits related to memory formation and storage, and its development, morphology, wiring, and function has been extensively studied. MBs consist of intrinsic Kenyon Cells that are divided into three major neuron classes (γ, α'/β' and α/β) and 7 cell subtypes (γd, γm, α'/β'ap, α'/β'm, α/βp, α/βs and α/βc) based on their birth order, morphology, and connectivity. These subtypes play distinct roles in memory processing, however the underlying transcriptional differences are unknown. Here, we used RNA sequencing (RNA-seq) to profile the nuclear transcriptomes of each MB neuronal cell subtypes. We identified 350 MB class- or subtype-specific genes, including the widely used α/β class marker Fas2 and the α'/β' class marker trio Immunostaining corroborates the RNA-seq measurements at the protein level for several cases. Importantly, our data provide a full accounting of the neurotransmitter receptors, transporters, neurotransmitter biosynthetic enzymes, neuropeptides, and neuropeptide receptors expressed within each of these cell types. This high-quality, cell type-level transcriptome catalog for the Drosophila MB provides a valuable resource for the fly neuroscience community.

KEYWORDS:

Drosophila; RNA-seq; mushroom body; transcriptome

PMID:
30397017
PMCID:
PMC6325895
DOI:
10.1534/g3.118.200726
[Indexed for MEDLINE]
Free PMC Article

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