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Nat Methods. 2018 Nov;15(11):932-935. doi: 10.1038/s41592-018-0175-z. Epub 2018 Oct 30.

Spatial organization of the somatosensory cortex revealed by osmFISH.

Author information

1
Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden. simone.codeluppi@ki.se.
2
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden. simone.codeluppi@ki.se.
3
Science for Life Laboratory, Stockholm, Sweden. simone.codeluppi@ki.se.
4
Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
5
Science for Life Laboratory, Stockholm, Sweden.
6
Faculty of Biotechnology and Food Engineering, Technion, Haifa, Israel.
7
Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.
8
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
9
Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden. sten.linnarsson@ki.se.
10
Science for Life Laboratory, Stockholm, Sweden. sten.linnarsson@ki.se.

Abstract

Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type-specific markers simultaneously on large tissue areas. Here, we describe a cyclic single-molecule fluorescence in situ hybridization methodology and define the cellular organization of the somatosensory cortex.

PMID:
30377364
DOI:
10.1038/s41592-018-0175-z

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