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Environ Sci Pollut Res Int. 2018 Dec;25(36):36545-36554. doi: 10.1007/s11356-018-3477-x. Epub 2018 Oct 29.

Heat processing effect of luteolin on anti-metastasis activity of human glioblastoma cells U87.

Author information

1
Faculty of Sciences of Tunis, University of Tunis El Manar, 2092, Tunis, Tunisia. elgueder.dorra@gmail.com.
2
Faculty of Dental Medicine, Unity of Bioactive and Natural Substances and Biotechnology UR17ES49, University of Monastir, Avicenne Street, 5000, Monastir, Tunisia. elgueder.dorra@gmail.com.
3
Faculty of Dental Medicine, Unity of Bioactive and Natural Substances and Biotechnology UR17ES49, University of Monastir, Avicenne Street, 5000, Monastir, Tunisia.
4
Faculty of Sciences of Tunis, University of Tunis El Manar, 2092, Tunis, Tunisia.
5
National School of Agronomy and Food Industries, Laboratory of Bimolecular Engineering, National Polytechnics Institute of Lorraine ENSAIA-INPL, 54505, Vandoeuvre les, Nancy, France.
6
Faculty of Pharmacy, Department of Pharmaceutical Sciences, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.
7
CNRS, Institut de Neurophysiopathologie, Aix Marseille University, Marseille, France.

Abstract

Among the flavonoïds, luteolin is a flavone that has been identified in many plants. It is known for its apoptotic potential with damage to DNA and cell cycle blockage. Many studies have shown that luteolin has anti-oxidant, anti-inflammatory, and anti-cancer activities. However, it is known that heat treatment (boiling, cooking, and treating with microwaves …) can influence the structure of flavonoïds, which often leads to changes in their activities. The present study was conducted to study the effect of heated luteolin on anti-tumor activity of glioblastoma cells U87. Glioblastoma cell viability was evaluated by MTT assay. Adhesion assay was performed on different protein matrices (collagen type 1, vitronectin, fibronectin, and poly-L-lysine); migration assay was determined by modified Boyden chambers and videomicroscopy, and finally, angiogenesis was tested in vitro by capillary network formation on Matrigel™. The results obtained show that the thermal treatment significantly reduces its cytotoxic activity and ability to inhibit cell adhesion to different protein matrices. It was also found that the heat processed significantly reduced the ability of luteolin to inhibit cell migration, cell invasion, and endothelial cell angiogenesis (HMEC-1). This suggests that heat treated luteolin has a lower anti-tumor potential than native luteolin. Graphical abstract ᅟ.

KEYWORDS:

Adhesion; Angiogenesis; Glioblastoma; Heat processing; Invasion; Migration

PMID:
30374718
DOI:
10.1007/s11356-018-3477-x
[Indexed for MEDLINE]

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