Format

Send to

Choose Destination
J Infect Dis. 2018 Oct 26. doi: 10.1093/infdis/jiy564. [Epub ahead of print]

Acquisition of Antibodies Against Endothelial Protein C Receptor-Binding Domains of Plasmodium falciparum Erythrocyte Membrane Protein 1 in Children with Severe Malaria.

Author information

1
Department of Medicine, The Peter Doherty Institute for Infection and Immunity, Parkville.
2
Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, Denmark.
3
School of Medicine and Pharmacology, University of Western Australia, Harry Perkins Research Institute, Fiona Stanley Hospital, Murdoch.
4
Papua New Guinea Institute of Medical Research, Madang.
5
Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Australia.
6
Department of Medical Biology, University of Melbourne, Parkville.
7
Walter and Eliza Hall Institute of Medical Research, Parkville.
8
Parasite and Insect Vectors Department, Institut Pasteur, Paris, France.

Abstract

Background:

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) mediates parasite sequestration in postcapillary venules in P. falciparum malaria. PfEMP1 types can be classified based on their cysteine-rich interdomain region (CIDR) domains. Antibodies to different PfEMP1 types develop gradually after repeated infections as children age, and antibodies to specific CIDR types may confer protection.

Methods:

Levels of immunoglobulin G to 35 recombinant CIDR domains were measured by means of Luminex assay in acute-stage (baseline) and convalescent-stage plasma samples from Papua New Guinean children with severe or uncomplicated malaria and in healthy age-matched community controls.

Results:

At baseline, antibody levels were similar across the 3 groups. After infection, children with severe malaria had higher antibody levels than those with uncomplicated malaria against the endothelial protein C receptor (EPCR) binding CIDRĪ±1 domains, and this difference was largely confined to older children. Antibodies to EPCR-binding domains increased from presentation to follow-up in severe malaria, but not in uncomplicated malaria.

Conclusions:

The acquisition of antibodies against EPCR-binding CIDRĪ±1 domains of PfEMP1 after a severe malaria episode suggest that EPCR-binding PfEMP1 may have a role in the pathogenesis of severe malaria in Papua New Guinea.

PMID:
30365003
DOI:
10.1093/infdis/jiy564

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center