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Nat Biotechnol. 2018 Oct 22. doi: 10.1038/nbt.4277. [Epub ahead of print]

De novo assembly of haplotype-resolved genomes with trio binning.

Author information

1
Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, Bethesda, Maryland, USA.
2
Institute of Medical Microbiology, Heinrich-Heine-University Düsseldorf, Düsseldorf, North Rhine-Westphalia, Germany.
3
Cell Wall Biology and Utilization Laboratory, ARS USDA, Madison, Wisconsin, USA.
4
Pacific Biosciences, Menlo Park, California, USA.
5
Davies Research Centre, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy SA, Australia.
6
Robinson Research Institute, The University of Adelaide, Adelaide SA, Australia.
7
US Meat Animal Research Center, ARS USDA, Clay Center, Nebraska, USA.

Abstract

Complex allelic variation hampers the assembly of haplotype-resolved sequences from diploid genomes. We developed trio binning, an approach that simplifies haplotype assembly by resolving allelic variation before assembly. In contrast with prior approaches, the effectiveness of our method improved with increasing heterozygosity. Trio binning uses short reads from two parental genomes to first partition long reads from an offspring into haplotype-specific sets. Each haplotype is then assembled independently, resulting in a complete diploid reconstruction. We used trio binning to recover both haplotypes of a diploid human genome and identified complex structural variants missed by alternative approaches. We sequenced an F1 cross between the cattle subspecies Bos taurus taurus and Bos taurus indicus and completely assembled both parental haplotypes with NG50 haplotig sizes of >20 Mb and 99.998% accuracy, surpassing the quality of current cattle reference genomes. We suggest that trio binning improves diploid genome assembly and will facilitate new studies of haplotype variation and inheritance.

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