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Small. 2018 Nov;14(45):e1803342. doi: 10.1002/smll.201803342. Epub 2018 Oct 11.

Patterned Optoelectronic Tweezers: A New Scheme for Selecting, Moving, and Storing Dielectric Particles and Cells.

Author information

1
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.
2
Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada.
3
Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3G9, Canada.
4
State Key Laboratory of Optoelectronic Materials and Technologies, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510275, China.
5
Institute for Electric Light Sources, Fudan University, Shanghai, 200433, China.
6
Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, M5S 3G4, Canada.
7
Department of Materials Science and Engineering, University of Toronto, Toronto, ON, M5S 3E4, Canada.
8
Photonics Group, Merchant Venturers School of Engineering, University of Bristol, Bristol, BS81UB, UK.
9
Medicine by Design, University of Toronto, Toronto, ON, M5S 3G9, Canada.
10
School of Biomedical Engineering, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.
11
Michael Smith Laboratories, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.

Abstract

Optical micromanipulation has become popular for a wide range of applications. In this work, a new type of optical micromanipulation platform, patterned optoelectronic tweezers (p-OET), is introduced. In p-OET devices, the photoconductive layer (that is continuous in a conventional OET device) is patterned, forming regions in which the electrode layer is locally exposed. It is demonstrated that micropatterns in the photoconductive layer are useful for repelling unwanted particles/cells, and also for keeping selected particles/cells in place after turning off the light source, minimizing light-induced heating. To clarify the physical mechanism behind these effects, systematic simulations are carried out, which indicate the existence of strong nonuniform electric fields at the boundary of micropatterns. The simulations are consistent with experimental observations, which are explored for a wide variety of geometries and conditions. It is proposed that the new technique may be useful for myriad applications in the rapidly growing area of optical micromanipulation.

KEYWORDS:

cell capture; dielectrophoresis; micropatterns; optical micromanipulation; optoelectronic tweezers

PMID:
30307718
DOI:
10.1002/smll.201803342

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