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Food Sci Biotechnol. 2017 Jul 20;26(4):1019-1028. doi: 10.1007/s10068-017-0143-x. eCollection 2017.

Response surface optimization for cellulose production from agro industrial waste by using new bacterial isolate Gluconacetobacter xylinus C18.

Author information

1
1Food Biotechnology Research Laboratory, Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal, Punjab 148 106 India.
2
2Department of Chemistry, Sant Longowal Institute of Engineering and Technology, Longowal, Punjab 148 106 India.

Abstract

In the present investigation, a low-cost medium prepared from molasses and corn steep liquor was used for the bacterial cellulose production by using an isolated bacterial strain. This bacterium, identified as Gluconacetobacter xylinus C18, was isolated from Indian fruit waste (rotten grapes). The process of cellulose production from the isolated bacterial strain was optimized using response surface methodology based on the central composite rotatable design. The optimum parameters for maximum bacterial cellulose production (4.34 g/L) obtained were sugarcane molasses concentration 10.77% (w/v) supplemented with 12.47% (v/v) corn steep liquor concentration at 31 °C, pH 6.5, and incubation time of 172 h. The structure of cellulose was characterized and confirmed by using SEM and FT-IR spectroscopy.

KEYWORDS:

Bacterial cellulose; Fourier transform infrared spectroscopy; Response surface methodology; Scanning electron microscopy

Conflict of interest statement

Compliance with ethical standardsAll authors declare that they have no conflict of interest.

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