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Development. 2018 Oct 26;145(20). pii: dev170100. doi: 10.1242/dev.170100.

Characterization of the ventricular-subventricular stem cell niche during human brain development.

Author information

1
Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT 06269, USA.
2
Department of Neurosurgery, Pediatrics, and Cellular & Molecular Physiology, Yale School of Medicine, New Haven, CT 06510, USA.
3
Department of Pathology, University of Manitoba, Winnipeg, R3E 3P5, Canada.
4
Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT 06269, USA joanne.conover@uconn.edu.

Abstract

Human brain development proceeds via a sequentially transforming stem cell population in the ventricular-subventricular zone (V-SVZ). An essential, but understudied, contributor to V-SVZ stem cell niche health is the multi-ciliated ependymal epithelium, which replaces stem cells at the ventricular surface during development. However, reorganization of the V-SVZ stem cell niche and its relationship to ependymogenesis has not been characterized in the human brain. Based on comprehensive comparative spatiotemporal analyses of cytoarchitectural changes along the mouse and human ventricle surface, we uncovered a distinctive stem cell retention pattern in humans as ependymal cells populate the surface of the ventricle in an occipital-to-frontal wave. During perinatal development, ventricle-contacting stem cells are reduced. By 7 months few stem cells are detected, paralleling the decline in neurogenesis. In adolescence and adulthood, stem cells and neurogenesis are not observed along the lateral wall. Volume, surface area and curvature of the lateral ventricles all significantly change during fetal development but stabilize after 1 year, corresponding with the wave of ependymogenesis and stem cell reduction. These findings reveal normal human V-SVZ development, highlighting the consequences of disease pathologies such as congenital hydrocephalus.

KEYWORDS:

Ependymogenesis; Human brain development; Stem cell niche; Ventricular-subventricular zone

PMID:
30237244
PMCID:
PMC6215394
[Available on 2019-10-15]
DOI:
10.1242/dev.170100
[Indexed for MEDLINE]
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