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Molecules. 2018 Sep 10;23(9). pii: E2305. doi: 10.3390/molecules23092305.

Phenethyl Isothiocyanate Inhibits In Vivo Growth of Xenograft Tumors of Human Glioblastoma Cells.

Chou YC1,2,3, Chang MY4, Lee HT5,6, Shen CC7,8, Harnod T9,10, Liang YJ11, Wu RS12,13, Lai KC14, Hsu FT15, Chung JG16,17.

Author information

1
Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 40754, Taiwan. chouycns@yahoo.com.tw.
2
Department of Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan. chouycns@yahoo.com.tw.
3
Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan. chouycns@yahoo.com.tw.
4
Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan. mchang@mail.tcu.edu.tw.
5
Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 40754, Taiwan. leesd2001@gmail.com.
6
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 11490, Taiwan. leesd2001@gmail.com.
7
Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 40754, Taiwan. shengeorge@yahoo.com.
8
Department of Physical Therapy, Hung Kuang University, Taichung 43302, Taiwan. shengeorge@yahoo.com.
9
Department of Neurosurgery, Hualien Tzu Chi General Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan. tomorha@yahoo.com.tw.
10
College of Medicine, Tzu Chi University 970, Hualien, Taiwan. tomorha@yahoo.com.tw.
11
Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 40754, Taiwan. shelby63liang@gmail.com.
12
Department of Anesthesiology, China Medical University Hospital, Taichung 404, Taiwan. rickwu@mail.cmuh.org.tw.
13
Department of Anesthesiology, China Medical University, Taichung 40402, Taiwan. rickwu@mail.cmuh.org.tw.
14
Department of Medical Laboratory Science and Biotechnology, College of Medicine and Life Science, Chung Hwa University of Medical Technology, Tainan 71703, Taiwan. kuangchi_lai@hotmail.com.
15
Departments of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan. sakiro920@mail.cmu.edu.tw.
16
Departments of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan. jgchung@mail.cmu.edu.tw.
17
Department of Biotechnology, Asia University, Taichung 413, Taiwan. jgchung@mail.cmu.edu.tw.

Abstract

Phenethyl isothiocyanate (PEITC) from cruciferous vegetables can inhibit the growth of various human cancer cells. In previous studies, we determined that PEITC inhibited the in vitro growth of human glioblastoma GBM 8401 cells by inducing apoptosis, inhibiting migration and invasion, and altering gene expression. Nevertheless, there are no further in vivo reports disclosing whether PEITC can suppress the growth of glioblastoma. Therefore, in this study we investigate the anti-tumor effects of PEITC in a xenograft model of glioblastoma in nude mice. Thirty nude mice were inoculated subcutaneously with GBM 8401 cells. Mice with one palpable tumor were divided randomly into three groups: control, PEITC-10, and PEITC-20 groups treated with 0.1% dimethyl sulfoxide (DMSO), and 10 and 20 μmole PEITC/100 μL PBS daily by oral gavage, respectively. PEITC significantly decreased tumor weights and volumes of GBM 8401 cells in mice, but did not affect the total body weights of mice. PEITC diminished the levels of anti-apoptotic proteins MCL-1 (myeloid cell leukemia 1) and XIAP (X-linked inhibitor of apoptosis protein) in GBM 8401 cells. PEITC enhanced the levels of caspase-3 and Bax in GBM 8401 cells. The growth of glioblastoma can be suppressed by the biological properties of PEITC in vivo. These effects might support further investigations into the potential use of PEITC as an anticancer drug for glioblastoma.

KEYWORDS:

apoptosis; caspase; glioblastoma; phenethyl isothiocyanate; xenograft

PMID:
30201893
PMCID:
PMC6225357
DOI:
10.3390/molecules23092305
[Indexed for MEDLINE]
Free PMC Article

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