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J Cell Sci. 2018 Oct 11;131(19). pii: jcs221648. doi: 10.1242/jcs.221648.

RSPH6A is required for sperm flagellum formation and male fertility in mice.

Author information

1
Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
2
Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
3
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
4
Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan ikawa@biken.osaka-u.ac.jp.
5
The Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.

Abstract

The flagellum is an evolutionarily conserved appendage used for sensing and locomotion. Its backbone is the axoneme and a component of the axoneme is the radial spoke (RS), a protein complex implicated in flagellar motility regulation. Numerous diseases occur if the axoneme is improperly formed, such as primary ciliary dyskinesia (PCD) and infertility. Radial spoke head 6 homolog A (RSPH6A) is an ortholog of Chlamydomonas RSP6 in the RS head and is evolutionarily conserved. While some RS head proteins have been linked to PCD, little is known about RSPH6A. Here, we show that mouse RSPH6A is testis-enriched and localized in the flagellum. Rsph6a knockout (KO) male mice are infertile as a result of their short immotile spermatozoa. Observation of the KO testis indicates that the axoneme can elongate but is disrupted before accessory structures are formed. Manchette removal is also impaired in the KO testis. Further, RSPH9, another radial spoke protein, disappeared in the Rsph6a KO flagella. These data indicate that RSPH6A is essential for sperm flagellar assembly and male fertility in mice.This article has an associated First Person interview with the first author of the paper.

KEYWORDS:

Axoneme; CRISPR/Cas9; Flagella; Radial spoke protein; Spermatozoa

PMID:
30185526
PMCID:
PMC6198453
[Available on 2019-10-01]
DOI:
10.1242/jcs.221648

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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